Caractérisation des abeilles sans aiguillon (Tetragonisca angustula) de l'Etat de Santa Catarina (Brésil) par l'ADN mitochondrial
Overview & Synopsis
The stingless bee Tetragonisca angustula is a meliponini bee naturally distributed from Argentina to Mexico. Morphologically, based on the mesepisternum color, it is separated into T. angustula angustula and T. angustula fiebrigi subspecies. The objective of this study was to characterize the restriction site variation in the mitochondrial DNA of each subspecies. Samples of worker bees were collected from two regions of Santa Catarina state, known as having a natural distribution of each subspecies. Each of the 138 colonies collected in the distribution region of T. a. angustula and of 72 colonies from the region of T. a. fiebrigi was individually analyzed. The ATPases 8, 6 and COIII mitochondrial genes were amplified and digested with seven restriction enzymes (PCR + RFLP). Large genetic differences were observed among bees collected in both geographical areas of natural distribution for the T. angustula subspecies. Four enzymes showed different restriction patterns that allowed separation of the subspecies.
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Document Information
| Document Title | Caractérisation des abeilles sans aiguillon (Tetragonisca angustula) de l'Etat de Santa Catarina (Brésil) par l'ADN mitochondrial |
|---|---|
| Author / Organization | Diego Fagundes Koling, Geraldo Moretto |
| Subject & Discipline | Multidisciplinary Academic Open Research |
| Categories | Science & Mathematics |
| Publication Year | 2010 |
| License / Rights | Open Access / Creative Commons |
| Resource Verification | Listed PDF (Verification Pending) |
| Original Host Website |
HAL Open Science
(hal.science)
|
| File Format | application/pdf (Portable Document Format) |
| Language | EN |
| Catalog ID | DOC-81188 |
| Original Source Link | Visit Source Page on hal.science » |