A live-imaging protocol to track cell movement in the Xenopus embryo
Overview & Synopsis
A live-imaging protocol to track cell movement in the Xenopus embryo Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.
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hal.science)
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Document Information
| Document Title | A live-imaging protocol to track cell movement in the Xenopus embryo |
|---|---|
| Author / Organization | Alexandre Chuyen, Fabrice Daian, Andrea Pasini, Laurent Kodjabachian |
| Subject & Discipline | Multidisciplinary Academic Open Research |
| Categories | Science & Mathematics |
| Publication Year | 2021 |
| License / Rights | Open Access / Creative Commons |
| Resource Verification | Listed PDF (Verification Pending) |
| Original Host Website |
HAL Open Science
(hal.science)
|
| File Format | application/pdf (Portable Document Format) |
| Language | EN |
| Catalog ID | DOC-255365 |
| Original Source Link | Visit Source Page on hal.science » |