Spectral and temporal distribution of biomolecules by Dynamic SERS

Overview & Synopsis

In this thesis, the definition of SERS as a biosensor has been tested and a new approach developed for. Also, in terms of quantification, it has been shown that SERS can be an efficient tool. Concerning the selectivity, the spectral quality was improved. A low limit of detection associated to the statistical and dynamic approach allows a very good sensitivity (under the nanomolar). This approach also enables a high reproducibility in time of the sensor. Thus, as low as SERS does not well answer to the sensor capabilities in a classical approach, in our case the coupling between a non-functionalized GNPs substrate coupled with a microfluidic chip, all mounted on a confocal microscope for temporal dynamic studies statistically analyzed has contributed to define SERS as an efficient biosensor.