Integrated microfluidic platform for single organoid culture and secreted extracellular vesicle isolation
Overview & Synopsis
In this abstract, we present a versatile microfluidic platform enabling the isolation of extracellular vesicles (EVs) secreted by a single organoid. Our automated device combines both the on-chip culture of a single organoid and an integrated micropump downstream allowing the continuous secretion sampling. Leveraging an immunomagnetic capture protocol, we achieve selective and efficient EVs isolation from whole secretions. This work is paving the way for an integrated companion test assay based on EVs secretion monitoring from organoids.
Indexed from repository: HAL Open Science
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hal.science)
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Document Information
| Document Title | Integrated microfluidic platform for single organoid culture and secreted extracellular vesicle isolation |
|---|---|
| Author / Organization | Marie Hut, Flora Clement, Frederic Bottausci, Josiane Denis, Manuel Alessio, François Boizot, Nadia Cherradi, Myriam Cubizolles, Fabrice Navarro, Yves Fouillet, Vincent Agache |
| Subject & Discipline | Multidisciplinary Academic Open Research |
| Categories | Science & Mathematics |
| Publication Year | 2024 |
| License / Rights | Open Access / Creative Commons |
| Resource Verification | Listed PDF (Verification Pending) |
| Original Host Website |
HAL Open Science
(hal.science)
|
| File Format | application/pdf (Portable Document Format) |
| Language | EN |
| Catalog ID | DOC-196143 |
| Original Source Link | Visit Source Page on hal.science » |