Validation of End-point and Real-Time PCR methods for the rapid detection of soy allergen in processed products

Overview & Synopsis

This work describes the development and validation of two PCR methods, End-point and real-time PCR for the detection of presence of soy protein in a wide rage of foodstuffs. Among the advantages of these techniques it is worth highlighting that these are reliable and sensitive, allowing to detect trace amounts of soybean in processed products. The TaqMan real-time PCR had the simplest, fastest testing process and high potential for automation, therefore representing the currently most suitable method for screening. Moreover, in order to verify the correct operation of the proposed methodology, the ELISA technique was used for quantitative determination of the soy protein. Also, thirty-five meat, fish and bakery processed products susceptible of containing soy which was not announced on the label were tested for the presence of soy DNA by using the methods proposed in. The methodologies can be useful in questions regarding the presence of soy protein in processed products, especially to verify the fulfilment of the labelling and security rules and to protect consumer's rights.