Basic Practical Microbiology

Overview & Synopsis

This PDF covers the
following topics related to Basic Practical Microbiology : The Basics, An
introduction to microbiology, aseptic technique and safety, Preparation, Safety
guidelines, Risk assessment, Good microbiological laboratory practice (GMLP),
Spillage management, Aerosols, Resources, Equipment, Apparatus, Materials,
Media, sterilisation and disinfection, Preparation of culture media, Pouring a
plate, Storage of media, Sterilisation vs disinfection, Sterilisation using the
autoclave or pressure cooker, Sterilisation of equipment and materials, Choice,
preparation and use of disinfectants, Inoculation and other aseptic procedures,
Essential points, Using a wire loop, Using a pipette, Flaming the neck of
bottles and test tubes, Working with bacteria and yeast Streak plate, Pour
plate, Using a spreader, Spread plate, Working with moulds, Incubation, In
conclusion: clearing up, Essential methods for maintaining, preparing and using
cultures Obtaining suitable cultures, Pure cultures, Maintaining stock cultures,
Checking cultures for contamination, Preventing contamination of cultures and
the environment, Aseptic transfer of cultures and sterile solutions, Preparing
cultures for class use, Microbiology in Action Practical activities, Testing
sensitivity to antimicrobial substances, Microscopy.