Academic Articles & Research Papers

Magnetic enrichment-coupled flow cytometry for highly sensitive detection of persistent CAR-T cells in spiked and clinical peripheral blood samples.

Calibrated flow cytometry enables rapid detection of CD45(-)EpCAM(+) extracellular vesicles for pancreatic cancer clinical assessment.

Protocol for FLOWMIST to profile autoreactivity of mouse monoclonal and serum antibodies by flow cytometry and microscopy.

Lysosome blockade induces divergent metabolic programs in macrophages and tumours for cancer immunotherapy

Abstract Background Platinum-drugs based chemotherapy in clinic increases the potency of tumor cells to produce M2 macrophages, thus leading to poor anti-metastatic activity and immunosuppression. Lysosome metabolism is critical for cancer ...

miR-130a-3p Exacerbates Sepsis-Induced Cardiac Dysfunction by Promoting Inflammation and Targeting PDK4

Background Sepsis is frequently complicated by systemic inflammatory injury and cardiac dysfunction, which serve as risk factors of adverse clinical outcomes and increased mortality risk. To clarify the function of miR-130a-3p in sepsis dia...

Flow cytometric resolution of yeast is affected by enzymatic treatment and culture media

Saccharomyces cerevisiae is an important model organism and a typical fungal representative for studies of eukaryotes. The cell cycle of yeast can be analyzed by flow cytometry, and refining the cytometric resolution of the cell cycle with ...

Role of hypothetical protein PA1-LRP in antibacterial activity of endolysin from a new Pantoea phage PA1

IntroductionPantoea ananatis has emerged as a significant plant pathogen affecting various crops worldwide, causing substantial economic losses. Bacteriophages and their endolysins offer promising alternatives for controlling bacterial infe...

Emodin, an Emerging Mycotoxin, Induces Endoplasmic Reticulum Stress-Related Hepatotoxicity through IRE1α–XBP1 Axis in HepG2 Cells

Emodin, an emerging mycotoxin, is known to be hepatotoxic, but its mechanism remains unclear. We hypothesized that emodin could induce endoplasmic reticulum (ER) stress through the inositol-requiring enzyme 1 alpha (IRE1α)–X-box-binding pro...

Characterization of antibiofouling behaviors of PVDF membrane modified by quaternary ammonium compound – combined use of QCM-D, FCM, and CLSM

In this study, we systematically evaluated the antibiofouling behavior of quaternary ammonium compound (QAC) blended polyvinylidene fluoride (PVDF) membrane using quartz crystal microbalance with dissipation monitoring (QCM-D) combined with...

The Protective Effect of Sulforaphane against Oxidative Stress through Activation of NRF2/ARE Pathway in Human Granulosa Cells

Objective: Sulforaphane (SFN) is a natural free radical scavenger that can reduce oxidative stress (OS) through mediating nuclear factor (erythroid-derived 2)-like 2 (NF-E2-related factor 2 or NRF2)/antioxidant response element (ARE) signal...

Data on the cytotoxicity of chlorogenic acid in 3D cultures of HT-29 cells

Functional foods, beyond basic nutrition, offer health benefits to consumers thanks to the presence of bioactive compounds such as some phytochemicals [1,2]. Today, these foods are of particular interest in biomedical research due to their ...

Accumulation of tissue-resident natural killer cells, innate lymphoid cells, and CD8+ T cells towards the center of human lung tumors

Lung cancer is a leading cause of cancer-related death worldwide. Despite recent advances in tissue immunology, little is known about the spatial distribution of tissue-resident lymphocyte subsets in lung tumors. Using high-parameter flow c...

Single Eye mRNA-Seq Reveals Normalisation of the Retinal Microglial Transcriptome Following Acute Inflammation

Background: Whether retinal microglia can maintain or restore immune homeostasis during and after inflammation is unclear. We performed single-eye mRNA-sequencing on microglia at different timepoints following a single inflammatory stimulus...

Deep Learning with Microfluidics for Biotechnology

Advances in high-throughput and multiplexed microfluidics have rewarded biotechnology researchers with vast amounts of data but not necessarily the ability to analyze complex data effectively. Over the past few years, deep artificial neural...

CViewer: a Java-based statistical framework for integration of shotgun metagenomics with other omics datasets

Abstract Background Shotgun metagenomics for microbial community survey recovers enormous amount of information for microbial genomes that include their abundances, taxonomic, and phylogenetic information, as well as their genomic makeup, t...