Academic Articles & Research Papers

Proteoliposomes – a system to study membrane proteins under buffer gradients by cryo-EM

Membrane proteins are vital to life and major therapeutic targets. Yet, understanding how they function is limited by a lack of structural information. In biological cells, membrane proteins reside in lipidic membranes and typically experie...

Mechanistic insights into TAM-mediated OMP assembly in Gram-negative bacteria

Summary: The β-barrel assembly machinery (BAM) is essential for outer membrane protein (OMP) biogenesis in Gram-negative bacteria and represents a validated target for antibiotic development. Its evolutionary relative, the translocation and...

The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism

A cryo-electron microscopy reconstruction of a stressosome complex from a Gram-negative bacterium, Vibrio vulnificus, reveals variations in subunit composition and symmetry, which could serve to adjust the activation threshold in the respon...

Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi

Abstract The mechanosensitive channel of small conductance (MscS) from E. coli (EcMscS) has served as the prevailing model system for understanding mechanotransduction in ion channels. Trypanosoma cruzi, the protozoan parasite causing Chaga...

Nucleosome Linker Length and Distribution as Major Players in Epigenetic Regulation: Insights from Cryo-Electron Microscopy and Modeling of Retina Cell Maturation.