Academic Articles & Research Papers

Protocol for precise large-region haplotype modification for isogenic human iPSC generation using CRISPR-Cas9.

CRISPR screens reveal MCT1-driven metabolic escape from SMAD3 inhibition as a therapeutic vulnerability.

CRISPR-Cas immune repertoires as an ecological record of bacterial interactions with mobile genetic elements in the human gut.

Mutation-induced penicillin binding protein 2A inactivation through CRISPR-dCas9 gene editing in methicillin-resistant S. aureus.

CRISPR/Cas9 for hepatitis B virus infection treatment

Abstract Hepatitis B virus (HBV) infection remains a global health challenge. Despite the availability of effective preventive vaccines, millions of people are at risk of cirrhosis and hepatocellular carcinoma. Current drug therapies inhibi...

SCC3 is an axial element essential for homologous chromosome pairing and synapsis

Cohesin is a multi-subunit protein that plays a pivotal role in holding sister chromatids together during cell division. Sister chromatid cohesion 3 (SCC3), constituents of cohesin complex, is highly conserved from yeast to mammals. Since t...

AGRICULTURE AND PLANT SCIENCE INNOVATIONS

Agriculture and Plant Science Innovations is a comprehensive and forward-looking resource that bridges traditional agricultural knowledge with modern scientific advancements. This book is designed to provide readers with a deep understandin...

The emerging and uncultivated potential of CRISPR technology in plant science

Phage Genetic Engineering Using CRISPR–Cas Systems

Since their discovery over a decade ago, the class of prokaryotic immune systems known as CRISPR–Cas have afforded a suite of genetic tools that have revolutionized research in model organisms spanning all domains of life. CRISPR-medi...

Progress and Perspective of CRISPR‐Cas9 Technology in Translational Medicine

Abstract Translational medicine aims to improve human health by exploring potential treatment methods developed during basic scientific research and applying them to the treatment of patients in clinical settings. The advanced perceptions o...

Use of CRISPR-Cas Technology in Medical Biotechnology

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology won the 2020 Nobel prize in chemistry. CRISPR was discovered in the genome of prokaryotes, where it functions as their adaptive immune system. E...

The Advancement of Medical Biotechnology in Developing Countries: Economic Opportunities and Challenges

Starting from decades ago, medical biotechnology has revolutionized the healthcare industry by offering new hopes for previously untreatable diseases and improving overall health outcomes. To explore the advancements in medical biotechnolog...

Current Evidence on Gene-Based and Epigenetic Therapeutic, Diagnostic, and Prognostic Strategies in Epithelial-Derived Carcinomas: A Scoping Review

Background: Epithelial-derived carcinomas exhibit genomic instability due to genetic mutation and epigenetic dysregulation. Mechanisms such as DNA methylation, histone modifications, and noncoding RNA regulation may lead to tumor progressio...

MEDICAL BIOTECHNOLOGY FOR DIAGNOSTICS AND THERAPEUTICS

Medical biotechnology is a fundamental area of modern healthcare, combining molecular biology, genetics, bioengineering, and computer sciences to improve disease diagnosis and treatment. Harnessing the power of biological systems and biomol...

Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a Mediated Multiplexable and Portable Detection Platform for GII Genotype Porcine Epidemic Diarrhoea Virus Rapid Diagnosis

Porcine epidemic diarrhoea virus (PEDV) is a member of the genus Alphacoronavirus in the family Coronaviridae. It causes acute watery diarrhoea and vomiting in piglets with high a mortality rate. Currently, the GII genotype, PEDV, possesses...

CircUCK2(2,3) promotes cancer progression and enhances synergistic cytotoxicity of lenvatinib with EGFR inhibitors via activating CNIH4–TGFα–EGFR signaling

Abstract Background Circular (circ)RNAs have emerged as crucial contributors to cancer progression. Nonetheless, the expression regulation, biological functions, and underlying mechanisms of circRNAs in mediating hepatocellular carcinoma (H...

Advances in CAR T cell therapy: antigen selection, modifications, and current trials for solid tumors

Chimeric antigen receptor (CAR) T cell therapy has revolutionized the treatment of hematologic malignancies, achieving remarkable clinical success with FDA-approved therapies targeting CD19 and BCMA. However, the extension of these successe...

Knockout of

Direct-seeded rice (DSR) offers a sustainable alternative for climate-resilient agriculture, but it is severely threatened by the root-knot nematode Meloidogyne graminicola . Knockout of susceptibility (S) genes has emerged as a promising s...

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology

Marek’s disease virus (MDV) is an important oncogenic α-herpesvirus that induces Marek’s disease (MD), characterized by severe immunosuppression and rapid-onset T-cell lymphomas in its natural chicken hosts. Historically, MD is regarded as ...

Co-Regulation of Protein Coding Genes by Transcription Factor and Long Non-Coding RNA in SARS-CoV-2 Infected Cells: An In Silico Analysis

Altered expression of protein coding gene (PCG) and long non-coding RNA (lncRNA) have been identified in SARS-CoV-2 infected cells and tissues from COVID-19 patients. The functional role and mechanism (s) of transcriptional regulation of de...