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Cracking rare disorders: a new minimally invasive RNA-seq protocol

Laurenz De Cock, Erika D’haenens, Lies Vantomme, Lynn Backers et al. • npj Genomic Medicine (2025)

Abstract RNA sequencing (RNA-seq) has become key to complementing exome and genome sequencing for variant interpretation. We present a minimally invasive RNA-seq protocol using short-term cultured peripheral blood mononuclear cells (PBMCs) ...

DOAJ Open Access (DOAJ) DOI: 10.1038/s41525-025-00502-7

BRI1 EMS SUPPRESSOR1 genes regulate abiotic stress and anther development in wheat (Triticum aestivum L.)

Dezhou Wang, Dezhou Wang, Jinghong Zuo, Jinghong Zuo et al. • Frontiers in Plant Science (2023)

BRI1 EMS SUPPRESSOR1 (BES1) family members are crucial downstream regulators that positively mediate brassinosteroid signaling, playing vital roles in the regulation of plant stress responses and anther development in Arabidopsis. Important...

DOAJ Open Access (DOAJ) DOI: 10.3389/fpls.2023.1219856

Fungal Communities Associated with Siricid Wood Wasps: Focus on <i>Sirex juvencus</i>, <i>Urocerus gigas</i>, and <i>Tremex fuscicornis</i>

Adas Marčiulynas, Jūratė Lynikienė, Artūras Gedminas, Aistė Povilaitienė et al. • Insects (2024)

We investigated the diversity and occurrence of wood wasps in Lithuania and determined communities of associated fungi. Trapping of wood wasps resulted in three different species, including Sirex juvencus, Urocerus gigas, and Tremex fuscico...

DOAJ Open Access (DOAJ) DOI: 10.3390/insects15010049

Chromosome-level genome assembly and population genomics analysis of Camellia rubituberculata provide insights into adaptation to karst habitats

Chao Yan, Ming-tai An, Ming Tang, Xin-xiang Bai et al. • Plant Diversity (2026)

Karst flora confined to isolated ‘habitat islands’ evolve specialized adaptations and unique traits, serving as ideal models for investigating adaptive evolution and species diversification mechanisms. Camellia rubituberculata, endemic to t...

DOAJ Open Access (DOAJ) DOI: 10.1016/j.pld.2025.09.004

The estimation of canopy attributes from digital cover photography by two different image analysis methods

Chianucci F, Chiavetta U, Cutini A • iForest - Biogeosciences and Forestry (2014)

Proximal sensing methods using digital photography have gained wide acceptance for describing and quantifying canopy properties. Digital hemispherical photography (DHP) is the most widely used photographic technique for canopy description. ...

DOAJ Open Access (DOAJ) DOI: 10.3832/ifor0939-007

Identifying strawberry DOF family transcription factors and their expressions in response to crown rot

Chun LUO, Yuyang HU, Bo SHU • Notulae Botanicae Horti Agrobotanici Cluj-Napoca (2022)

Crown rot is one of the most destructive diseases of cultivated strawberry. The DOF family transcription factors, which involved in biotic stress, has not been studied in responding to strawberry crown rot. In this study, the DOFs of Fragar...

DOAJ Open Access (DOAJ) DOI: 10.15835/nbha50112640

Evolutionary Analysis of Four Recombinant Viruses of the Porcine Reproductive and Respiratory Syndrome Virus From a Pig Farm in China

Jiankui Liu, Jiankui Liu, Jiankui Liu, Liling Lai et al. • Frontiers in Veterinary Science (2022)

The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens causing substantial economic losses to the Chinese swine industry. In this study, we analyzed the complete genome sequences of four PRRSV...

DOAJ Open Access (DOAJ) DOI: 10.3389/fvets.2022.933896

Physical and Chemical Effects of Different Working Gases in Coffee Brewing: A Case Study of Caffè Firenze

Giulia Angeloni, Piernicola Masella, Lorenzo Guerrini, Agnese Spadi et al. • Foods (2020)

(1) Background: Recently, a new espresso extraction method, Caffè Firenze, has been developed, which uses gas at operating pressures of 20 bar to obtain abundant, persistent foam. The experiment aimed to evaluate the effect of using six gas...

DOAJ Open Access (DOAJ) DOI: 10.3390/foods9121825

Inhibition of Citrus Huanglongbing Disease by <i>Paenibacillus polymyx</i> KN-03 and Analysis with Transcriptome and Microflora

Yuehua Yang, Fangkui Wang, Jialin Jiang, Ling Jiang • Agronomy (2023)

Soil drench treatment using Paenibacillus polymyxa strain KN-03 was applied to citrus plants infected with Candidatus Liberibacter asiaticus (CLas). The infection status was assessed using PCR and a real-time quantitative PCR detection syst...

DOAJ Open Access (DOAJ) DOI: 10.3390/agronomy13122958

Bone Diagenesis in Short Timescales: Insights from an Exploratory Proteomic Analysis

Noemi Procopio, Caley A. Mein, Sefora Starace, Andrea Bonicelli et al. • Biology (2021)

The evaluation of bone diagenetic phenomena in archaeological timescales has a long history; however, little is known about the origins of the microbes driving bone diagenesis, nor about the extent of bone diagenesis in short timeframes—suc...

DOAJ Open Access (DOAJ) DOI: 10.3390/biology10060460

Analysis of Cathinone Alkaloids in Khat by HPLC-Q-TOF In-source Fragmentation Multistage Mass Spectrometry

SHI Hong-fei1, XU Bo-peng1, QUAN Yang-ke2, XU Cheng-xin1, ZHOU Xiu-qi1 • Zhipu Xuebao (2024)

Khat is a kind of common drug plant. Many experiments have shown that the content of cathinone norpseudoephedrine and norephedrine in some varieties of Khat are similar. However, some studies showed that there are differences in the intensi...

DOAJ Open Access (DOAJ) DOI: 10.7538/zpxb.2023.0065

Forensic Discrimination of White Architectural Paints via ATR FT-IR Spectroscopy and Chemometrics

Sweety Sharma, Gurpreet Kaur, Chongtham Nimi, Rajinder Singh • Arab Journal of Forensic Sciences & Forensic Medicine (2024)

The present work entailed the comparative examination and discrimination of white architectural paint samples of different brands/manufacturers. The analysis of this evidence can facilitate exhibiting a linkage between the victim, and the c...

DOAJ Open Access (DOAJ) DOI: 10.26735/GCNC4529

miR-3113-5p, miR-223-3p, miR-133a-3p, and miR-499a-5p are sensitive biomarkers to diagnose sudden cardiac death

Fengping Yan, Yuanyuan Chen, Xing Ye, Fu Zhang et al. • Diagnostic Pathology (2021)

Abstract Background Sudden cardiac death (SCD) remains a great health threat and diagnostic challenge, especially those cases without positive autopsy findings. Molecular biomarkers have been urgently needed for the diagnosis of SCD display...

DOAJ Open Access (DOAJ) DOI: 10.1186/s13000-021-01127-x

Comparative analysis of chemical toxicological studies of pregabalin in neuropathic pain management (review)

Nargiz Parkhatkyzy, Alma Shukirbekova, Zumrad Usmanalieva, Kundyzay Mirzakir et al. • Journal of Pharmacy & Pharmacognosy Research (2026)

Context: The use of pregabalin in neuropathic pain management has been increasingly associated with concerns regarding toxicity, particularly in cases of overdose, misuse, and co-exposure with central nervous system depressants. In parallel...

DOAJ Open Access (DOAJ) DOI: 10.56499/jppres_14.3.2550

Simultaneous analysis of creatinine and uric acid in human urine samples using mean centering, ratio subtraction, and derivative spectrophotometric techniques as a green analytical protocol

Dashty K. Ali, Hemn A. Qader, Nabil A. Fakhre • Green Analytical Chemistry (2025)

Four rapid, accurate, and straightforward derivative spectrophotometric methods were developed to quantify a binary mixture of creatinine (CRT) and uric acid (UA) in human urine samples. The first technique was based on a zero-crossing tech...

DOAJ Open Access (DOAJ) DOI: 10.1016/j.greeac.2025.100289

Forensic Application of Next Generation Sequencing Technology in the Typing of Y Chromosome Genetic Marker

YANG Yue , TAO Rui-yang , LI Min , et al. • Fayixue Zazhi (2021)

The paternal inheritance characteristics of Y chromosome have been widely used in the forensic genetics field to detect the genetic markers in the non-recombining block, and used in the studies such as, genetic relationship identification, ...

DOAJ Open Access (DOAJ) DOI: 10.12116/j.issn.1004-5619.2020.501205

Optimization of Bacillus cereus Fermentation Process for Selenium Enrichment as Organic Selenium Source

Xujun Chen, Shuyi Li, Xin Cong, Xin Cong et al. • Frontiers in Nutrition (2020)

Selenium is an essential trace element and micronutrient for human health. Application of organic selenium in plants and microorganisms as trace element supplement is attracting more and more attention. In this study, Bacillus cereus, an im...

DOAJ Open Access (DOAJ) DOI: 10.3389/fnut.2020.543873

Toward 3D reconstruction of damaged vehicles for investigating traffic accidents in Thailand using a photogrammetric approach

Polpreecha Chidburee, Sunanta Sriphannam, Chattichai Waisurasingha • Engineering and Applied Science Research (2022)

Traffic accident investigation by the police forensic science department in Thailand is essential to determine their causes. The collection of forensic evidence from damaged vehicles after an accident plays a significant role in damage asse...

DOAJ Open Access (DOAJ)

UPLC-MS/MS Metabolomics Reveals Babao Dan’s Mechanisms in MASH Treatment with Integrating Network Pharmacology and Molecular Docking

Shijiao Zhang, Yanding Su, Ao Han, He Qi et al. • Pharmaceuticals (2025)

Background: Metabolic dysfunction-associated steatohepatitis (MASH) is a progressive disease that easily develops into cirrhosis and hepatocellular carcinoma, but its pathogenesis is not clear, and most therapeutic drugs have obvious limita...

DOAJ Open Access (DOAJ) DOI: 10.3390/ph18081111