Computational optical streak microscopy of megahertz acoustic microbubble dynamics

Abstract Real-time dynamic imaging of microbubbles is crucial for understanding their microscale biophysical interactions and advancing ultrasound therapy. Despite progress in time-resolved optical imaging, existing techniques still face trade-offs between acquisition speed, spatial resolution, affordability, and system complexity. Here, we introduce compressed optical-streaking dark-field ultrahigh-speed microscopy (COSDUM), a compact imaging platform that synergistically combines compressed sensing, streak imaging, dark-field microscopy, and deep learning. COSDUM compressively records megahertz acoustic microbubble dynamics over a wide field of view in a snapshot and reconstructs spatially resolved dynamics using a convolutional neural network-based algorithm. Using COSDUM, we captured stable cavitation, nonlinear oscillations, post-excitation free oscillations, and inertial collapse across microbubbles whose radii range from 0.5 to 2.1 μm. Applying COSDUM to microbubble-cell interaction in whole blood, we observed, for the first time, interplay between vibrating microbubbles and blood cells, including microbubble-driven platelet dynamics and highly asymmetric microbubble deformation and conformation around an adjacent red blood cell.

# Applied optics. Photonics # Ultrahigh-speed microscopy # Computational imaging # Deep learning # Acoustic microbubble dynamics # Microbubble-cell interactions

Miguel Marquez, Yingming Lai, Miao Liu, et al. (2026). "Computational optical streak microscopy of megahertz acoustic microbubble dynamics". PhotoniX. https://doi.org/10.1186/s43074-026-00232-8